C4118
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 | Immunoblotting Tissue extracts and whole cell lysates were separated on SDS-PAGE and blotted with Anti-CRMP1 (N-terminal) (Cat. No. C4118). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution 1:3,000 Lanes 1,5: Rat brain (S1 fraction) 2: Mouse brain (S1 fraction) 3: HEK-293T cells ransfected with human CRMP1 4: Untransfected HEK-293T cells 5: Inhibition with CRMP1 immunizing peptide (10 μg/mL) |
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C4118
Sigma
Anti-CRMP1 (N-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-Collapsin Response Mediator Protein 1, Anti-Dihydropiraminidase-like 1 (DPYSL1), Anti-Dihydropyriminidase Related Proterin-1 (DRP1, DRP-1) |
Description
| Immunogen | synthetic peptide corresponding to amino acids 174-190 of human CRMP1, conjugated to KLH. This sequence is identical in mouse and rat CRMP1. |
| Physical form | Solution in 0.01 M phosÂphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~62 kDa |
| species reactivity | human, rat, mouse |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting: 0.5-1 μg/mL using HEK-293T cells expressing human CRMP1, mouse brain, and rat brain extracts (S1 fraction) |
| Gene Information | human ... CRMP1(1400) |
| rat ... Crmp1(25415) | |
| mouse ... Crmp1(12933) | |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Related product | C2868, Anti-CRMP1 antibody produced in rabbit |
